In Naujas, change the first few lines to:
function Naujas( img )
% imshow(img);
i.e. pass the image in as an argument instead of calling your function.
In test pushbutton2_Callback, add the line:
Naujas( image1 );
I haven't looked at any of what the rest of the code does to see whether there are other problems, but that will let you pass the image variable to your function.
