清空筛选条件
清空筛选条件

primer design example - why is scoring matrix changed?

1 次查看（过去 30 天）
Ekaterina Avershina
Ekaterina Avershina 2016-11-13
I need to design long primers of around 50 bp; and i follow the 'Exploring primer design' example (https://se.mathworks.com/help/bioinfo/examples/exploring-primer-design.html). When checking for cross-hybridization, the scoring matrix is changed from NUC44 to scr_mat = [-1,-1,-1,1;-1,-1,1,-1;-1,1,-1,-1;1,-1,-1,-1;]. I have 3 questions: (1) why? what do these numbers mean? (2) are these numbers specific for 18-30 bp, or are they valid for longer oligos? and (3) In this example, 'good' pair is the one with scr <=3, why? what does it mean? Is that 3 or less than 3 nucleotides hybridizing?

请先登录，再回答此问题。

回答（0 个）

类别

在 Help Center 和 File Exchange 中查找有关 Bioinformatics Toolbox 的更多信息

标签

产品

Community Treasure Hunt

Find the treasures in MATLAB Central and discover how the community can help you!

Start Hunting!

Translated by