If the object you are looking for is very bright compared to the background, I would just use an intensity threshold to find spots (if you have a color camera - consider converting the image to black and white by just summing up all three color channels) like
img_binary=img>threshold;
After that you can easily find the positions where your bright spots are. Consider the functions bwlabel (that will group all connected "ones" in your binary matrix img_binary). Now you could just take the mean position of the identified groups by bwlabel to get the positions of your two spots. If your image is too noisy - consider imdilate before running bwlabel.
