Doubts about image processing

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Daniel 2011-3-17

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Hello,

I am doing a research where i have to work with images from the corneal nerves, and they are really dark. These pictures where taken by a confocal microscopy. I am having difficulties to divide the pictures in blocks. I really need to do this because i want to apply the histogram to small regions and then do the segmentation. Can anyone help me?

Thank you for your time.

Daniel Carvalho

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Jan 2011-3-17

Could you explain the difficulties you have with any details? What exactly means "really dark" - are they completely black? What kind of images are you working with? Can you post a small example of the images and the code?

Daniel 2011-4-6

the images that i am working is something like this http://www.iovs.org/content/48/5/2017/F1.small.gif.

I want to isolate the nerves (the white stripes). I am using filters like the median, the functions imadjust, adapthisteq, bwmorph, rangefilt...

I can't show you my code because it is really confusing.

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Answer 1

Sean de Wolski 2011-4-6

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Perhaps Brett Shoelson's local Otsu utility would help you:

http://www.mathworks.com/matlabcentral/fileexchange/29764-thresholdlocally

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Answer 2

Tim Zaman 2011-4-6

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I would recommend using photoshop (or equivalent) first to edit the image to a usable format, as photoshop in that matter is more user-friendly, though less scientific.