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How can I save this .tiff file correctly?

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Merrilee Thomas
Merrilee Thomas 2018-3-20
评论： Walter Roberson 2018-3-20
The code below is the code I wrote to control our microscope and led lights using serial commands.
I am running into a problem with saving it however, the image collected is not being saved correctly. HELP!
The area with the double stars (bold) is where I wrote the code to save our image, and take user input to save the image.
best
Merrilee
%first set up channel....
obj = instrfind('Port', 'COM3')
if isempty(obj)
obj = serial('COM3');
else
fclose(obj);
obj = obj(1)
end
fopen(obj);
set(obj, 'BaudRate', 115200);
set(obj, 'DataTerminalReady', 'off');
set(obj, 'FlowControl', 'hardware');
set(obj, 'Terminator', {10,10});
fprintf(obj, 'M 0');
data = query(obj, 'L? 0');
% this will set current for channel 1
% and the "L" is the limit.
fprintf(obj, 'L 0 700');
currentlimit = query(obj, 'L? 0');
% this will set current for channel 2
fprintf(obj, 'L 1 700');
currentlimit = query(obj, 'L? 1');
% to set or change "brightness Mode" also must turn off LED first before
% changing brightness if doing this in a command line.
fprintf(obj, 'BP 0 100');
brightnessvalue = query(obj, 'BP? 0');
% this will change brightness for channel 2
fprintf(obj, 'BP 0 100');
brightnessvalue = query(obj, 'BP? 0');
% this is the quickest on/off switch(470)
fprintf(obj, 'O 0 1'); %on
fprintf(obj, 'O 0 0'); %off
% and this turns on and off channel 2(560)!!!
fprintf(obj, 'O 1 1') % on
fprintf(obj, 'O 1 0') % off
% now set up imaging....
%vid = videoinput('hamamatsu', 1, 'MONO16_BIN2x2_1024x1024_FastMode');
vid = videoinput('hamamatsu', 1, 'MONO16_BIN4x4_512x512_FastMode');
src = getselectedsource(vid);
%vid.FramesPerTrigger = 10;
% TriggerRepeat is zero based and is always one
% less than the number of triggers.
%vid.TriggerRepeat = 2;
src.ExposureTime = .7;
%when I want to take multiple images, wait awhile and then takee more
%images in the same file.
for i = 1:2
vid.FramesPerTrigger = 10;
fprintf(obj, 'O 0 1'); %on
preview(vid);
%fprintf(obj, 'O 0 1'); %on
start(vid),wait(vid);
for l = 1:2
fprintf(obj, 'O 0 0'); %off
pause (10);
end;
end;
stoppreview(vid);
% below is where my code isn't saving the image correctly - the .tiff file that saves has no data (i think) saved to %it. it just looks like a pixel landscape.
alpha=input('What do you want to name you file? ','s')
lambda = input('Variable ?', 's')
x = getdata(vid);
filename = alpha
RE = lambda
save([filename '.tif'], 'RE');
clear lambda;
closepreview(vid);
delete(vid);
clear vid;

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回答（1 个）

Walter Roberson
Walter Roberson 2018-3-20
Use imwrite() or the tiff class to save tif files. save() is for saving as .mat files.
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Merrilee Thomas
Merrilee Thomas 2018-3-20
ok. I rewrote the code to imwrite(x, [filename '.tif']); however, now I get an error that says 4-D data not supported for TIFF files. what do i need to change. I need the images I am taking to be in a tiff format. best, Merr
Walter Roberson
Walter Roberson 2018-3-20
There are a lot of examples in the imwrite documentation. Just pass in RE and then the file name.

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