You can define the path of a file to access it:
Folder = 'C:\Temp\';
FileList = dir(fullfile(Folder, '*.jpg'));
for iFile = 1:numel(FileList)
File = fullfile(FileList(iFile).folder, FileList(iFile).name);
% Do what you want with this file, e.g.:
Img = imread(File)
end
