Tortuosity of blood vessels

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Hello,
I am trying to calculate the Tortuosity of blood vessels. (Vessel tortuosity is calculated as the sum of branch lengths divided by the sum of the euclidean distance between their end points).
I have the following questions:
  1. How to calculate the lengths of both actual branches and the imaginary straight lines between nodes
  2. How do I mark the vessel branches (orange) and the branch nodes (yellow) as shown in the picture.
  3. I am calculating "spinelength" as the sum of all pixels. How do I calculate individual branch lengths?
  4. Any suggestions in preprocessing would be appreciated.
clc;
clear;
close all
% Read the image
I=imread('VAD.png');
figure,imshow(I)
%convert it to gray scale
I_gray=rgb2gray(I);
%Sharpen the image
b = imsharpen(I_gray,'Amount',8);
h = fspecial('average', [3 3]);
b = imfilter(b, h);
%choose brighter objects
Bina=b>150
figure,imshow(Bina);
se = strel('cube',3)
erodedBW = imerode(Bina,se);
%Remove small objects from binary image
BW2 = bwareaopen(Bina,100)
figure,imshow(BW2);
skelImage = bwskel(BW2, 'MinBranchLength', 10);
MinBranchLength = round(sum(skelImage(:))/2)
skelImage = bwskel(BW2,'MinBranchLength',MinBranchLength);
figure,imshow(skelImage)
endpointImage = bwmorph(skelImage, 'endpoints');
[rows, columns] = find(endpointImage)
spineLength = sum(skelImage(:))
straightLineDistance = sqrt((columns(2) - columns(1))^2 + (rows(2) - rows(1))^2)
tortuosity = spineLength / straightLineDistance

采纳的回答

Image Analyst
Image Analyst 2021-2-11
Call bwmorph() to get the branchpoints then use that to erase the branch points. Then label each curve and call bwmorph() to get the endpoints to get the straight line distance. Pretty easy, in fact you've probably already done it by now. You might have gotten something like (untested):
bp = bwmorph(mask, 'branchpoints');
mask(bp) = false; % Erase branchpoints.
[labeledImage, numRegions] = bwlabel(mask);
for k = 1 : numRegions
thisRegion = ismember(labeledImage, k);
endpoints = bwmorph(thisRegion, 'endpoints');
% Get coordinate
[r, c] = find(endpoints);
euclideanDistance(k) = sqrt((r(end)-r(1)).^2 + (c(end)-c(1)).^2);
area(k) = sum(thisRegion);
tortuosity(k) = area(k) / euclideanDistance(k);
end
How does that compare to your code?

更多回答(1 个)

darova
darova 2021-2-9
Since you already have a skeletonized image
  • dilate your image at nodes to separate each lines
BW1 = imdilate(BW,zeros(3)); % dilate image with matrix 3x3
  • use bwlabel to separate each line

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