How to identify and locate cells

Question

AAS 2021-2-19

0
链接

此问题的直接链接

https://ww2.mathworks.cn/matlabcentral/answers/750404-how-to-identify-and-locate-cells

回答： yanqi liu 2021-2-20

在 MATLAB Online 中打开

I have processed the following image and need to identify the cells- location of cells and the count.

Once identified, I would like to fill the cells .

            I=imcomplement(imflatfield(rgb2gray(imread('well1.png');
            I-imgaussfilt(I,50);
            imshow(I)

Because some are clumped, I did use the watershed algorithm but it did not work the way I liked. The image is below. I want the cell lining to be complete for all of them.

            L = watershed(I);
            Lrgb = label2rgb(L);
            imshow(Lrgb)
            imshow(imfuse(bw,Lrgb))
            bw2 = ~bwareaopen(~bw, 10);
            imshow(bw2)
            D = -bwdist(~bw);
            imshow(D,[])
            Ld = watershed(D);
            imshow(label2rgb(Ld))
            Ld = watershed(D);
            imshow(label2rgb(Ld))
            bw2 = bw;
            bw2(Ld == 0) = 0;
            imshow(bw2)
            mask = imextendedmin(D,2);
            imshowpair(bw,mask,'blend')
            D2 = imimposemin(D,mask);
            Ld2 = watershed(D2);
            bw3 = bw;
            bw3(Ld2 == 0) = 0;
            imshow(bw3)